Multiple enzyme immobilization using heat-sensitive polymeric nanocarrier for enhanced cascade reaction
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초록
내용
Various enzymes have been studied for the application and one of the applications is immobilization. As enzyme is immobilized on support matrix, it can drive enhanced stability and easy downstream process. Moreover, multiple enzyme immobilization can help construction of cascade reaction to enhance production yield. However, it is difficult to immobilize multiple enzymes while maintaining high immobilization yield, retention of enzymatic activity and low leaching. Furthermore, multiple enzyme immobilization considering with reaction rate is hard because of difference of immobilization yield of each enzyme. In this study, we demonstrated that heat-sensitive polymeric nanocarrier (HSPC) has desirable feature for immobilization in case of single/multiple enzymes. Since HSPC can reduce size when temperature is increased, enzymes can be located inside HSPC after temperature treatment. We have demonstrated that various enzymes were immobilized in HSPC while maintaining desirable features.