Human BBB model for study for BBB penetration of drugs
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초록
내용
Microfluidic biomimetic system represents a new avenue in a range of area in preclinical precision drug validation and new drug discovery. A patient’s own cells could be adapted with induced pluripotent stem cell (iPSC) techniques to develop biomimetic system which emulates individual organ-level function. Here, I will discuss my recent research related to organ mimetics based on the stem cell technology and microfluidics. The ‘organ-on-a-chip’ and ‘organoid’ models of the human blood-brain barrier (BBB) are lined by iPSC-derived brain microvascular endothelium, primary human brain astrocytes and pericytes that recapitulate the high level of barrier function of the in vivo human BBB. The endothelium expresses high levels of tight junction proteins, multiple functional efflux pumps, and transcytosis pathways, which enable reliable monitoring of transport of drugs and therapeutic antibodies across the human BBB in vitro and screen the BBB permeable drugs.