Silk Fibroin Microspheres Prepared in Macromolecular Crowding Conditions
발표자
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초록
내용
In intracellular environments, various macromolecules, such as protein and nucleic acid, are presented at a high concentration. In such macromolecular crowding conditions, the protein folding is more efficient than in dilute conditions. Silk protein (SF) is well known for its biocompatibility and biodegradability having potential for the drug delivery system. In this study, we prepared the silk fibroin microsphere via slow acidification by adding glucono-delta-lactone (GDL). As silk fibroin forms coacervate in poly(vinyl alcohol) (PVA) solution, the beta-sheet transition of SF occurs while maintaining coacervate, and SF microspheres were formed. The coacervates and microspheres were investigated by fluorescence microscopy and scanning electron microscopy (SEM). And for drug release characterization, UV-visible absorbance was performed.