Sustained Release of Reactive Oxygen Species via<EM> in situ</EM> Cross-linking Gelatin Hydrogels Attenuates Tonsil-derived Mesenchymal Stem Cells Senescence by Redistribution of Glucose-regulated Protein
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내용
Accumulating evidence suggests that reactive oxygen species (ROS) is toxic by-products of aerobic metabolism that are harmful to living cells, cell death and senescence. Hence, the low level of stress produced by ROS can promote the cell proliferation and control senescence. In this regard, we examined the association of mesenchymal stem cells (MSC) senescence with sustained ROS release in biomimetic environmental culture using ROS releasing in situ forming hydrogel system using tonsil-derived MSC (TMSC). We divided experimental groups into two groups as follows; young TMSC (3 ~ 8p) and old TMSC (15 ~ 25p), individually. Decreased protein expression of glucose-regulated protein (GRP) in old TMSC, an ER stress-related factor, was recovered with mild-ROS environment by sustained ROS releasing 3D culture condition. Here, we suggest that the mild level of ROS releasing hydrogel system can control TMSC senescence as well as ER stress of TMSC by regulation of the GRP protein expression and intra/extracellular distribution.