Modular Protective Nanoporous Polyurea Network Membranes for Enzymatic Catalysis
발표자
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초록
내용
Immobilization in porous solid supports are commonly employed for enzymes, while various nanoporous structures have shown additional benefits such as shielding against deactivation agents. However, a few reports employed bicontinuous nanoporous membrane for enzyme protection and efficient enzymatic functions simultaneously. Recently, we reported caging lipase in nanoporous covalent polymer membrane prepared from the poly (ethylene glycol)/poly-urea network mixtures. Facile membrane preparation and enzyme loading by filtration and improved lifespan compared to free enzyme. To broaden the scope of the enzymes and examine the protective effects of confinement on the activity of enzymes, we studied the enzymatic reactions by other categories of enzyme. The effect of confinement on enzymatic kinetics and shielding efficiency were thoroughly investigated to prove the general applicability of caging in the bicontinuous nanoporous structure.