Regenerative medicine with stem cell, reprogramming, and tissue engineering can provide highly effective therapeutics for treating incurable diseases. Several engineering approaches have been employed to improve regenerative medicine. In particular, functional biomaterials such as hydrogels can enhance the efficacy of regenerative medicine by providing tissue-mimetic biochemical and biophysical cues. Herein, biomimetic hydrogel platforms are demonstrated to improve stem cell engineering, reprogramming and organoid technology. Acknowledgment: This work was supported by a grant (2018M3A9H1021382) from the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (MSIT), Korea.