Spatiotemporal proximity crosslinking by light activation (Spotlight) for in vivo interactome mapping
발표자
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초록
내용
Proximity labeling is a recently developed method that enables proteomic profiling of specific regions in living cells. However, the diffusive character of in situ-generated reactive species by proximity labeling enzymes cannot be used easily to identify physically interacting partners with the protein of interest (POI). In the present study, we developed an in vivo proteome mapping method that enables spatiotemporal proximity crosslinking by a genetically targeted light-activated ligand of the HaloTag protein in a live mammalian cell (Spotlight). Spotlight was used to reveal the unknown interaction partner of a newly identified mitochondrial protein, exonuclease 3′-5′ domain containing 2 (EXD2) in mammalian cells.