ROS-responsive and PD-L1 targeted Thioketal Nanoparticles Triggered Mitochondrial-mediated Apoptotic Cell Death in Melanoma Model
발표자
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초록
내용
Melanoma (MEL) is characterized by high PD-L1 expression and hypoxic tumor environment. Hypoxic condition promotes reactive oxygen species (ROS) production and this could be a target for MEL. In this study, we have prepared PD-L1 targeted and temozolomide-loaded ROS-responsive thioketal nanoparticles (anti-PD-L1-TMZ-TKNPs) for treatment of MEL. Anti-PD-L1-TMZ-TKNPs was successfully constructed with the particle size of 103.2 ± 1.6 nm and zeta potential of -12.9 ± 0.3 mV. The release study showed that 80% of TMZ was released at 48 h from anti-PD-L1-TMZ-TKNPs with 1 mM H2O2 at acidic pH suggested the ROS-responsive behavior. In B16F10 xenograft mice model, maximum distribution with a significant decrease in tumor volume was observed after intravenous injection of anti-PD-L1-TMZ-TKNPs. In conclusion, anti-PD-L1-TMZ-TKNPs offers the targeted and ROS-responsive release of TMZ for treatment of MEL.