Activation of NK cell Functionality by Coacervate-mediated Interleukin-15 Delivery
발표자
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초록
내용
A successful delivery of IL-15 to natural killer (NK) cells and a subsequent activation of NK cells’ anticancer efficacy could be a promising immuno-cancer therapy. For the protection of IL-15 with maintaining its bioactivity, coacervate (a complex (1) methoxy polyethylene glycol-poly(ethylene arginyl aspartate diglyceride) (mPEG-PEAD), a cationic polymer, (2) heparin, anionic counterpart, and (3) cargo IL-15)) were developed. Release profile of IL-15 could be controlled by varying PEG chain length of mPEG-PEAD. Proliferation rate of NK cells was enhanced by coacervate-mediated IL-15 delivery. During 4 hr of co-cultured NK and MCF-7 with IL-15, the bolus group more killed MCF-7 than coacervate groups. Also, bolus group produce more interferon-γ than coacervate group. Although IL-15 did not release sufficiently from coacervate in the short-term culture, it is expected that IL-15 released sufficiently in the long-term culture, which could lead to better results than the bolus group.