COPPER-FREE CLICKED NANOCLUSTERS OF GOLD NANOPARTICLES AND QUANTUM DOTS FOR IN VIVO REAL-TIME IMAGING AND TUMOR SENSING.
발표자
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초록
내용
Nowadays, fabrication of biosensors for cancer detection, monitoring of angiogenesis or cancer metastasis and cancer imaging holds vast potential. In this study, a biosensor based on clustering of matrix metallopeptidase (MMP) enzyme responsive gold nanoparticles (AuNP) and quantum dots (QD) by azide-alkyne copper-free click reaction was proposed. Briefly, AuNP were surface-decorated with two different types of poly(ethylene glycol) (PEG). Carboxylate-PEG was used to conjugate with MMP cleavable peptide (MMP CP) and azide-PEG was used for azide-alkyne copper-free click reaction between AuNP and alkyne decorated QD to form clusters at tumor site. As a result, FRET occurred during the clustering process and fluorescence intensity of QD was quenched. Thereby, our MMP-responsive nanoclusters could be used for in vivo real-time imaging as well as sensing tumor-specific environments.