Recently, tissue adhesive sealants have been used to treat damaged tissue or to replace conventional suture technology in the treatment of diseases requiring surgery. Existing tissue adhesive sealants are mostly toxic or at risk of viral infection. Therefore, it is necessary to develop a sealant having excellent adhesion and no toxicity. In this study, biocompatibility, biodegradability, and tissue adhesion materials using natural polymers are investigated. Natural polymers are suitable materials for tissue-adhesive and biodegradable hydrogels, but it is disadvantageous in that they possess weak mechanical properties. By controlling the structure of the hydrogel made of natural polymer, it has sufficient strength and adhesion as a sealant. In addition, mechanical properties and structural differences according to their solvents were also investigated.