Fabrication and evaluation of in situ biodegradable pullulan hydrogels as an injectable hemostatic agent
발표자
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초록
내용
Injectable, biodegradable pullulan hydrogels offer multiple attractive benefits. In this study, the enzyme-mediated injectable pullulan hydrogels containing human plasma components like thrombin were fabricated to use as a hydrogel type hemostatic agent. Its hydrogel has many advantages such as biocompatibility, flexibility, and blood coagulability. The modified pullulan was characterized by ATR-FTIR, 13C NMR and 1H NMR to confirm introduction of tyramines. Gelation, rheological properties were decided as a function of concentration of horseradish peroxidase and pullulan solutions. The in vivo animal testing showed that the modified hydrogel considerably reduced clotting time compared to the unmodified hydrogel. The modified pullulan hydrogel containing blood coagulation agents has adjusted gelation time, biodegradability, hemostatic ability as an effective hemostatic agent of massive blood flow through blood vessels or body parts.