Highly purified MSC-derived exosome isolation methods for attenuating inflammatory cells
발표자
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초록
내용
Extracellular vesicles (EVs, including exosomes) derived from mesenchymal stem cells (MSCs-EVs) have been studied as vital components for cell-based regenerative medicine and attenuating inflammation. Typically, various isolation methods have been developed for increasing yield. Moreover, the components of cell culture medium have been considered to get clinically active purified EVs. In order to obtain high yield EVs, we compared ultracentrifuge-based method and tangential flow filtration (TFF) system-based method. And the bioactivity according to the impurities from fetal bovine serum (FBS)-derived exosomes has been compared depending on exosome depletion methods from FBS. To demonstrate the biological properties, depending on purity of EVs, we pretreated EVs to inflammation induced cells. The effect of EVs was compared in vitro using various inflammation-related assays. These studies indicate that the purity of MSC exosomes is an important factor for efficient biological effects.