Vascularized cardiac tissue network by a direct patterning via a 3D bioprinter
발표자
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초록
내용
3D bioprinting is a widely used technology to dispense cell-laden biomaterials for rapid fabrication of complex 3D tissue constructs or artificial organs. In this study, we developed heterogeneous cell-laden microchannel network using human umbilical vein endothelial cell-cardiomyocytes by a direct patterning with a 3D bioprinter. This system also facilitated cell alignment and migration within each constructs and promoted vascular network with high cell viability. Calcium imaging was used to optically probe intracellular calcium ion signals during excitation-contraction coupling in cardiomyocytes within 3D vascular network. The cell-laden 3D constructs could be extended to serve as in vitro models for clinical cardiovascular disease researches and cardiovascular tissue regenerations.