Lectin-Janus nanoparticles for the exosome-mediated diagnosis of metastatic pancreatic cancer
발표자
()
초록
내용
This study introduces the pancreatic cancer cell-derived exosome detection technology, which is based on the specific binding of lectins to distinctive glycan profiles on the surface of exosomes. Lectins with a high and specific affinity for sialic acid or fucose were attached to bifunctional Janus nanoparticles (JNPs), which facilitated interactions with pancreatic cancer cell-derived exosomes in a microfluidic device. Here, we show that pancreatic cancer cell-derived exosomes from two cell lines and plasma samples collected from patients diagnosed with pancreatic cancer were successfully captured on the lectin-conjugated JNPs with affinities that were comparable to those of CD19-9, a conventional antibody. In addition, exosome detection using our platform was able to differentiate between metastatic and nonmetastatic pancreatic cancer cells.