POS8-0887
Polyphenol-Based Biointerfaces for Selective Lipoprotein Interference Control in Complex Biofluids
Topic
S8. Frontiers of Functional Polymers in Biology and Medicine
When and Where
Sep 30, 2026
08:30 - 09:30
Room 301 (Grand Ballroom)
Session Chairs
Heesuk KIM
Jinhye BAE
Presenter(s)
Nayoung Son (DGIST)
Co-Author(s)
Abstract
Extracellular vesicles (EVs) have attracted considerable interest as biomarkers for liquid biopsy, driving extensive efforts to improve their isolation from complex biological fluids such as human plasma. However, even after conventional purification procedures, residual biomolecules in the sample can interfere with downstream molecular analysis, reducing analytical reliability. In particular, low-density lipoproteins (LDL) remain a major source of interference due to their high abundance and physicochemical similarity to EVs.
Here, we investigated polyphenol-based biointerfaces as a surface chemistry for EV analysis. Epigallocatechin gallate (EGCG)-modified biointerfaces markedly reduced LDL signals while maintaining representative EV markers. These interfaces modulate interfacial interactions to selectively suppress lipoprotein-related interference. Mechanistic characterization suggested that this selective behavior is associated with distinct interfacial interactions involving polyphenolic functional groups. Similar attenuation was also observed with catechol- and gallol-containing polyphenols, suggesting a shared structural origin. Furthermore, selective attenuation of LDL interference remained consistent in human plasma-derived samples.
Importantly, EV molecular signatures were preserved after EGCG treatment, indicating that lipoprotein interference can be selectively minimized during analysis without compromising EV-derived molecular information. This work broadens the application of polyphenol-based surface chemistry for the design of functional biointerfaces in biosensing, molecular diagnostics, and related biomedical technologies.
Here, we investigated polyphenol-based biointerfaces as a surface chemistry for EV analysis. Epigallocatechin gallate (EGCG)-modified biointerfaces markedly reduced LDL signals while maintaining representative EV markers. These interfaces modulate interfacial interactions to selectively suppress lipoprotein-related interference. Mechanistic characterization suggested that this selective behavior is associated with distinct interfacial interactions involving polyphenolic functional groups. Similar attenuation was also observed with catechol- and gallol-containing polyphenols, suggesting a shared structural origin. Furthermore, selective attenuation of LDL interference remained consistent in human plasma-derived samples.
Importantly, EV molecular signatures were preserved after EGCG treatment, indicating that lipoprotein interference can be selectively minimized during analysis without compromising EV-derived molecular information. This work broadens the application of polyphenol-based surface chemistry for the design of functional biointerfaces in biosensing, molecular diagnostics, and related biomedical technologies.
