POS9-0817
Fabrication of Dual-DNA-Functionalized Janus Particles via Organic Emulsion-Based Masking
Topic
S9. Polymer Technology for Sustainability
When and Where
Sep 30, 2026
08:30 - 09:30
Room 301 (Grand Ballroom)
Session Chairs
Heesuk KIM
Jinhye BAE
Presenter(s)
Keonwoo Lee (POSTECH)
Co-Author(s)
Abstract
Janus particles with spatially separated surface functionalities are attractive building blocks for programmable colloidal assembly, biosensing, and sequence-specific molecular recognition. In this study, we present a simple strategy for fabricating dual-DNA-functionalized Janus particles using organic emulsion-based masking. Particles were partially embedded at the interface of organic emulsion droplets, where the organic phase acted as a temporary mask to protect one side of the particle surface. The exposed region was selectively functionalized with the first DNA strand, followed by removal of the organic mask and subsequent modification of the previously protected region with a second DNA strand. This sequential masking and functionalization process enabled the formation of Janus particles bearing two distinct DNA populations on opposite surface domains. Fluorescence microscopy confirmed the spatial separation of the two DNA-functionalized regions, demonstrating successful anisotropic surface modification. Compared with conventional planar substrate-based masking methods, the emulsion-based approach offers a facile and potentially scalable route for producing DNA-patterned colloidal particles. These dual-DNA-functionalized Janus particles could serve as versatile platforms for DNA-mediated particle self-assembly and biosensing applications based on sequence-specific molecular recognition.
