ORGS2-0012
Investigations into Biodistribution of Nanoparticles using Microfluidic in 3D Vitro Models
Topic
GS2. Graduate Student Oral Session II: Functional Biomaterials and Cosmetic Polymer Engineering
When and Where
Sep 28, 2026
15:12 - 15:24
Room 102
Session Chairs
Chaenyung CHA
Ilkoo NOH
Jun Shik CHOI
Presenter(s)
Zeyan Xu (University of New South Wales)
Co-Author(s)
Abstract
Brain cancer is one of the deadliest diseases in the world due to the blood-brain barrier which blocks the drugs from reaching the target tissue. With the discovery of the glucose transporter 1 (GLUT-1) receptors and its high expression level in blood brain barrier (BBB). Targeting GLUT-1 holds great promise in drug delivery for brain cancer treatment. However, synthesizing different types of suitable GLUT-1 targeting nanoparticles with the ideal size for drug delivery remains to be a challenge. What’s more, A 3D-Bioprinted Vascularized Glioblastoma-on-a-Chip is designed as a vitro BBB model for investigating the biodistribution of several nanoparticle system.
Therefore, there are two designed different nanoparticle systems for drug delivery: Glyconanoparticle and Polymeric Lipid Nanoparticle. Glyconanoparticle consists of cationic glycopolymer and negatively charged drug (DNA/SiRNA) self-assembling via microfluidic chips. Polymeric Lipid Nanoparticle consists of a lipid core (negatively charged polymer DNA and cationic phospholipid DODMA) and a polymeric shell (amphiphilic polymer) self-assembling via micromixer (Figure 1B). In the present work, Temozolomide (TMZ), which is a chemotherapy drug for brain tumours, is encapsulated by amphiphilic polymer via micromixer. The cytotoxicity of the nanoparticles is tested using Human Umbilical Vein Endothelial Cells (HUVECs), which is used to coat the vessel channel in the BBB model. Finally, the biodistribution of the TMZ nanoparticle is investigated in this in vitro BBB model.
Therefore, there are two designed different nanoparticle systems for drug delivery: Glyconanoparticle and Polymeric Lipid Nanoparticle. Glyconanoparticle consists of cationic glycopolymer and negatively charged drug (DNA/SiRNA) self-assembling via microfluidic chips. Polymeric Lipid Nanoparticle consists of a lipid core (negatively charged polymer DNA and cationic phospholipid DODMA) and a polymeric shell (amphiphilic polymer) self-assembling via micromixer (Figure 1B). In the present work, Temozolomide (TMZ), which is a chemotherapy drug for brain tumours, is encapsulated by amphiphilic polymer via micromixer. The cytotoxicity of the nanoparticles is tested using Human Umbilical Vein Endothelial Cells (HUVECs), which is used to coat the vessel channel in the BBB model. Finally, the biodistribution of the TMZ nanoparticle is investigated in this in vitro BBB model.
