DNA Origami Lattice-Assisted Fabrication of Patchy Gold Nanoparticles

In this study, we present a DNA nanostructure-assisted strategy for the spatial control of surface functionalization on gold nanoparticles. Gold nanoparticles were positioned within a designed DNA-based framework, where selective surface modification and subsequent particle release could be induced by external DNA inputs. Through this process, directionally defined interaction sites were introduced onto the nanoparticle surface, enabling controlled assembly through complementary DNA-mediated interactions. The resulting nanoparticles showed the ability to form programmed assemblies depending on the designed surface configuration. In addition, the structural stability of the DNA framework after particle release suggests its potential use as a reusable template for nanoparticle processing. Overall, this work demonstrates that DNA-based nanostructures can serve as a useful platform for controlling nanoparticle surface anisotropy and guiding programmable colloidal assembly.