POS8-0525
Polymeric Nanoparticles Containing Lumogen® Dyes for Cellular Detection and Imaging
Topic
S8. Frontiers of Functional Polymers in Biology and Medicine
When and Where
Oct 1, 2026
08:30 - 09:30
Room 301 (Grand Ballroom)
Session Chairs
Jinkee HONG
Ki Su KIM
Presenter(s)
Sujin Jeong (Pukyong National University)
Co-Author(s)
Abstract
Lumogen fluorescent dyes have great light stability as well as high fluorescence quantum yields. Despite the excellent photophysical properties of dyes, the highly hydrophobic structure in common makes the compounds difficult to function as biological fluorescence probes. To generally utilize a family of organic fluorescent dyes such as Lumogen Red, Orange, Yellow, and Violet, as a biomarker for cellular imaging in flow cytometry and fluorescence microscopy, we used an encapsulation method using amphiphilic block copolymer namely Pluronic F127. The photostability of Lumogen nanoparticles were compared with commercial dye, Alexa488 and DAPI, used primarily bioimaging. Cell uptake of the probe nanoparticles by HeLa cells was analyzed by flow cytometry and confocal laser scanning microscopy. Also, we observed to confirm the position of the Lumogen nanoparticles in the HeLa cells using a high voltage electron microscope.
