ORGS2-0478
Maskless Projection Lithography of Protein Gradients on Tunable Antifouling Polymeric Coatings
Topic
GS2. Graduate Student Oral Session II: Functional Biomaterials and Cosmetic Polymer Engineering
When and Where
Sep 28, 2026
16:00 - 16:12
Room 102
Session Chairs
Chaenyung CHA
Ilkoo NOH
Jun Shik CHOI
Presenter(s)
Hanyan Lyu (University of Freiburg, Faculty of Engineering)
Co-Author(s)
Abstract
BioBitmaps (Digital Biological Bitmaps) is a manufacturing platform that transcodes digital image information across scales into spatial distributions of biological function. Using a passivated, antifouling interface with ultra-low background noise as a "physical canvas", the platform employs controlled light patterns (e.g., via Digital Micromirror Devices or scanning laser beams) as "digital brushes" to trigger photo-initiated coupling reactions in situ and covalently anchor biomolecules (e.g., proteins, peptides) onto the surface. By linearly mapping pixel grayscale to biomolecular surface density, BioBitmaps enables high-fidelity fabrication of patterned biointerfaces with robust stability and continuous biofunctional gradients at resolutions ranging from micrometers to submicrometers.
The BioBitmaps technology relies on three essential components: photo-initiated coupling, digitally controlled light patterns, and biofunctionalizable yet strongly antifouling substrate coatings as the background to pattern. While extensive toolboxes exist for the first two components, coatings that combine robust biofunctionalization with outstanding antifouling performance remain a major challenge.
Here, we address this challenge by employing CHic (C,H-insertion crosslinking) chemistry to fabricate multifunctional copolymer coatings from three complementary monomer classes: photoactive monomers (e.g., benzophenone-containing monomers) for UV-induced crosslinking and covalent immobilization, hydrophilic/antifouling monomers for ultra-low nonspecific adsorption, and functional monomers bearing addressable groups for post-functionalization. By tuning composition and interfacial properties, the coatings provide a versatile, stable, and antifouling BioBitmap surface for high-fidelity biomolecular photopatterning. This polymer-based strategy extends BioBitmaps toward programmable biointerfaces for applications in biomaterials, biosensing, microfluidics, and organ-on-a-chip systems.
The BioBitmaps technology relies on three essential components: photo-initiated coupling, digitally controlled light patterns, and biofunctionalizable yet strongly antifouling substrate coatings as the background to pattern. While extensive toolboxes exist for the first two components, coatings that combine robust biofunctionalization with outstanding antifouling performance remain a major challenge.
Here, we address this challenge by employing CHic (C,H-insertion crosslinking) chemistry to fabricate multifunctional copolymer coatings from three complementary monomer classes: photoactive monomers (e.g., benzophenone-containing monomers) for UV-induced crosslinking and covalent immobilization, hydrophilic/antifouling monomers for ultra-low nonspecific adsorption, and functional monomers bearing addressable groups for post-functionalization. By tuning composition and interfacial properties, the coatings provide a versatile, stable, and antifouling BioBitmap surface for high-fidelity biomolecular photopatterning. This polymer-based strategy extends BioBitmaps toward programmable biointerfaces for applications in biomaterials, biosensing, microfluidics, and organ-on-a-chip systems.
