INIDS2-1585
Development of a Stratum Corneum Lipid Membrane Model for Assessing Liposomal Transdermal Delivery
When and Where
Nov 30, -0001
00:00 - 00:00
Room 102
Presenter(s)
Jae Jung Kim (Hongik University)
Co-Author(s)
Abstract
In cosmetics, establishing a reliable skin screening platform is essential for evaluating skin permeability and elucidating transdermal delivery mechanisms. Although excised human skin remains the gold standard, its practical application is severely constrained by ethical issues, limited availability, and high costs. Meanwhile, existing synthetic membranes often fail to replicate the complex nanoscale lipid packing structures of native skin. To address these challenges, we developed a stratum corneum (SC) lipid membrane model which reproduces human barrier properties while offering a cost-effective, high-throughput tool for mechanistic studies of skin penetration enhancers.
Through a deposition and annealing process of ceramides, free fatty acids, cholesterol, and cholesterol sulfate onto porous substrates, this platform enabled precise control over lipid packing structures. As confirmed by ATR-FTIR and wide-angle X-ray scattering (WAXS), varying the fatty acid chain length successfully generated either an orthorhombic (OR) structure corresponding to healthy skin or a hexagonal (HEX) structure mimicking impaired barriers. Furthermore, Franz diffusion studies showed that the model exhibits high quantitative correlations with excised human SC permeation parameters (i.e., permeability coefficient and lag time). Most importantly, post-permeation analyses of liposomal active delivery demonstrated an enhanced lipid orthorhombicity, providing key physical evidence for a vesicle-lipid fusion mechanism driven by cholesterol integration. Overall, this platform offers insights into liposome-skin interactions and supports the rational design of advanced transdermal formulations.
Through a deposition and annealing process of ceramides, free fatty acids, cholesterol, and cholesterol sulfate onto porous substrates, this platform enabled precise control over lipid packing structures. As confirmed by ATR-FTIR and wide-angle X-ray scattering (WAXS), varying the fatty acid chain length successfully generated either an orthorhombic (OR) structure corresponding to healthy skin or a hexagonal (HEX) structure mimicking impaired barriers. Furthermore, Franz diffusion studies showed that the model exhibits high quantitative correlations with excised human SC permeation parameters (i.e., permeability coefficient and lag time). Most importantly, post-permeation analyses of liposomal active delivery demonstrated an enhanced lipid orthorhombicity, providing key physical evidence for a vesicle-lipid fusion mechanism driven by cholesterol integration. Overall, this platform offers insights into liposome-skin interactions and supports the rational design of advanced transdermal formulations.
