POS4-1375
A Self-Assembled Particle Platform for Low-Cost DNA Sequencing
Topic
S4. Colloids, Interfaces, and Molecular Assemblies for Functional Soft Materials
When and Where
Sep 29, 2026
08:30 - 09:30
Room 301 (Grand Ballroom)
Session Chairs
Hae Jung SON
Boseok KANG
Presenter(s)
Jaeyoung Kim (POSTECH)
Co-Author(s)
Abstract
DNA sequencing has become a cornerstone technology in precision medicine, life sciences, and biotechnology, yet its cost remains high due to expensive sequencing reagents and single-use patterned flow cells. Here, we present a particle-based sequencing platform that addresses both limitations. Instead of being generated directly on the flow cell, DNA clusters are formed on particles that self-assemble into a close-packed two-dimensional monolayer on an unpatterned surface through programmable DNA hybridization. This strategy enables ultrahigh-density cluster arrays that increase sequencing throughput while reducing reagent consumption, and it eliminates the need for costly patterned flow cells. Furthermore, the reversible nature of DNA hybridization allows the particles to be removed after sequencing, enabling reuse of the flow cell. We demonstrate sequencing-by-synthesis on particle-bound DNA clusters together with DNA-guided two-dimensional particle self-assembly, establishing a foundation for a scalable and cost-effective next-generation sequencing platform.
