POS8-1276
Effects of single-segmented nanofibers in composite spheroids and their effect on bio-activities
Topic
S8. Frontiers of Functional Polymers in Biology and Medicine
When and Where
Oct 1, 2026
08:30 - 09:30
Room 301 (Grand Ballroom)
Session Chairs
Jinkee HONG
Ki Su KIM
Presenter(s)
Bada Shin (Department of Chemical Engineering (BK21 FOUR), Dong-A University)
Co-Author(s)
Abstract
While 3D stem cell spheroids enhanced maintenance of stemness, efficiency of differentiation, and protein production, limited mass diffusion induced severe inner hypoxia. In this study, we biomimetically engineered polydopamine-coated poly(L-lactic acid) single-segmented fibers (P-SFs, ~68 µm), and size-controlled hybrid spheroids were made by assembling various amount of human adipose-derived stem cells with the fibers.
In vitro analyses showed that the fibers were homogeneously bound to cell membranes, significantly enhancing core infiltration and preventing apoptosis while preserving the stemness. Depending on the size of spheroids, the larger spheroid secreted the more angiogenic proteins, and the smaller spheroid exhibited the more facilitated osteogenic differentiation. Moreover, the composite spheroid composed with 10 μg of fibers and 40,000 cells of fibroblasts showed the more release of extracellular vesicle (EV) by loosening the dense and compact surficial structure of cell only spheroid.
The results demonstrated that the hybridization of P-SFs in a spheroid circumvented the diffusion limitation and moderated the dense cell-cell interaction of spheroid enabled strategic modulation of stem cell fate and large-scale production of EV.
In vitro analyses showed that the fibers were homogeneously bound to cell membranes, significantly enhancing core infiltration and preventing apoptosis while preserving the stemness. Depending on the size of spheroids, the larger spheroid secreted the more angiogenic proteins, and the smaller spheroid exhibited the more facilitated osteogenic differentiation. Moreover, the composite spheroid composed with 10 μg of fibers and 40,000 cells of fibroblasts showed the more release of extracellular vesicle (EV) by loosening the dense and compact surficial structure of cell only spheroid.
The results demonstrated that the hybridization of P-SFs in a spheroid circumvented the diffusion limitation and moderated the dense cell-cell interaction of spheroid enabled strategic modulation of stem cell fate and large-scale production of EV.
