RNA Folding and the Non-equilibrium Action of RNA Chaperones
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초록
내용
In cells, RNA chaperones assist misfolding-prone ribozymes to fold correctly, so that the ribozymes can carry out their self-splicing activity. CYT-19 is an ATP-consuming RNA chaperone that accelerates the production of native group I intron ribozyme by partially unfolding the kinetically trapped structures. Using the theoretical framework based on the iterative annealing mechanism, we establish that to maximize the processing of pre-RNA, an optimal balance should exist between the timescales of self-splicing activity and CYT-19-mediated production of the native ribozyme. We found that the activity of CYT-19 is optimized to unfold the misfolded structures but is not so high that it disrupts the native ribozyme, which ensures that the yield of the self-splicing reaction is maximized.