Gradient hydrogel with fibroblast spheroids for full-thickness skin regeneration
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초록
내용
Hydrogel-based scaffolds have been widely investigated in tissue engineering to accelerate tissue regeneration. However, replicating the physiological microenvironment of tissues with appropriate biological cues remains а significant challenge. In this work, we present the integration of L929 mouse fibroblast spheroids into gradient hydrogels to mimic the dermal stiffness microenvironment and investigate its impact on full-thickness skin regeneration. Here, we present the gradient hydrogel via photocrosslinking and the gradient hydrogel is characterized in vitro and assessed the feasibility of wound healing application. The prepared gradient hydrogel shows noticeable wound healing effect and minimal scar formation. Taken together, this gradient hydrogel, which reproduces the physical gradients of skin, is biocompatible and can be used as a potential candidate for excellent regeneration of full thickness skin loss.