Evaluating the Low Molecular Weight β-Glucan as an Immunomodulatory Adjuvant in Dendritic Cells
발표자
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초록
내용
β-Glucans, polysaccharides from mushrooms, yeasts, oats, barley, and seaweed, have diverse biological activities and potential healthcare applications. This study aims to evaluate the efficacy of low molecular weight β-glucans (LMG) as immunomodulatory adjuvants. Produced with β-glucan phosphorylase and primarily consisting of megalooligosaccharides, LMG was tested on dendritic cells. It significantly increased co-stimulatory molecules CD86, CD80, and I-A/I-E upon OVA stimulation. The effects on CD86 and CD80 were similar to the adjuvant QS-21, while its enhancement of I-A/I-E was more pronounced. LMG also induced TNF-α, IL-6, and Th1 cytokine IL-12p40 productions more than QS-21, with no significant change in Th2 cytokine IL-4. LMG induced CD8+ T cell proliferation comparable to QS-21. These results suggest LMG is a promising immunomodulatory adjuvant with greater efficacy than current options.