Barley β-glucan Decorated Nanocomplexes for Macrophage Targeting and M1 Polarization
발표자
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초록
내용
In this study, bG/CpG/Ag nanocomplexes were developed to target macrophages and induce their M1 polarization. Barley β-glucan (bG) interacts with the dectin-1 receptor on macrophages, while ROS from Ag nanoparticles and CpG oligonucleotide (CpG ODN) enhance immune activation. Ag NPs were synthesized via Ag⁺ ions reduction and subsequently coated with CpG ODN. Finally, periodate-oxidized bG was conjugated with CpG ODN, forming bG/CpG/Ag nanocomplexes through imine bond formation. The nanocomplexes showed 150~200 nm diameter and negative zeta-potential with high serum stability and could release CpG in intracellular reducing condition with low cytotoxicity. Enhanced cellular uptake and intracellular trafficking of the nanocomplexes were observed in RAW264.7 cells, demonstrating high targeting ability. The nanocomplexes generated ROS and increased M1 marker genes expression (iNOS and COX) without affecting M2 markers (Arg-1 and TGF-β), indicating its efficacy in inducing M1 polarization.