Hyaluronic acid/calcium phosphate hybrid gene delivery system for in vitro osteogenic differentiation of human mesenchymal stem cells
발표자
()
초록
내용
Human mesenchymal stem cells (hMSCs) have been studied for decades in regenerative medicine and tissue engineering. Despite their self-renewal and multipotent properties, their unpredictable behavior in vivo hinders their application in the clinic. In this study, biocompatible calcium phosphate (CaP)-based nanoparticles stabilized with a catechol-derivatized hyaluronic acid (dopa-HA) conjugate were proposed as a carrier for gene transfection into hMSCs to enhance osteogenic differentiation. Since HA specifically binds to the CD44 receptor expressed on bone marrow-derived hMSCs, dopa-HA/CaP significantly increased the transfection efficiency in hMSCs compared to branched polyethyleneimine (bPEI, MW 25 kDa) without any cytotoxicity. Simultaneous delivery of plasmid DNA encoding bone morphogenetic protein 2 (BMP-2 pDNA) and microRNA 148b (miRNA-148b) by dopa-HA/CaP enhanced BMP-2 expression and suppressed noggin, an antagonist of BMP-mediated osteogenic differentiation.