Nucleic acid-based nanostructures for cell cryopreservation
발표자
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초록
내용
DNA-based self-assembly technology has enabled the precise design of nanostructures with different shapes and functions. Here, we demonstrate that self-assembled DNA origami nanostructures have a substantial ability to protect cells undergoing freeze-thaw cycles and therefore can be used as cryoprotectant agents. Sheet-like, rectangular DNA origami structures ('DNA nanopatches') showed high cell viability at much lower working concentrations compared to dimethyl sulfoxide (DMSO), with minimal cytotoxicity. By optimizing the shape of the DNA origami nanostructures, the cytoprotective performance of the structure could increase further. Inhibition of ice crystal growth in both intra- and extracellular regions and the protection of cell membranes during cryopreservation were key points for cell survival. This finding is expected to deepen the fundamental understanding of cell preservation under freezing environment and impact current cryopreservation technologies.