Cryoprotective Dendritic Polyglycerols for Cells and Their Network in Media
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초록
내용
Antifreeze proteins protect organisms from freezing damage by inhibiting ice formation. Ice recrystallization inhibition (IRI) of various small molecules and polymers has been extensively investigated. In our previous study, we demonstrated the effect of degree of branching of polyglycerols on IRI behavior including hyperbranched (hbPG) and linear polyglycerol (linPG). However, it has limitations that have been identified only in pure water. Herein, we synthesized PGs with various topologies and evaluated their IRI activity in buffer systems. Interestingly, dendritic polyglycerols (dPGs) exhibited superior IRI activity compared to other counterparts. Molecular dynamic simulations were employed to investigate the ice growth rate of PGs. Cryopreservation using dPG exhibited a significantly improved cell viability compared to conventional 10% DMSO. Finally, the cryopreservation of HUVEC cells demonstrated the successful preservation of their cellular functionality after thawing.