Hyaluronic acid-based microneedle for extended preservation and transdermal delivery of extracellular vesicles
발표자
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초록
내용
The use of extracellular vesicles (EVs) holds potential for disease diagnosis and treatment. Nevertheless, their clinical application faces challenges due to the poor long-term stability of EVs and ineffective delivery methods. This research investigated the potential for clinical use of EVs by incorporating human adipose stem cell-derived EVs (ADSC-EVs) into hyaluronic acid-based dissolving microneedles (EV@MN). The ADSC-EVs retained their biological functions for over six months under mild storage conditions, particularly at temperatures below 4°C. Additionally, the EV@MN enabled precise and convenient intradermal delivery, providing a sustained release of ADSC-EVs to dermal fibroblasts. The release of EVs facilitated the synthesis of extracellular matrix proteins like collagen and elastin, enhanced fibroblast proliferation, and regulated fibroblast phenotype. This study proposes a feasible and effective formulation for the clinical application of ADSC-EVs.