Systemic delivery of protein nanoparticles to cells effectively prevents acetaminophen-induced liver toxicity and hepatotoxicity
발표자
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초록
내용
Direct protein delivery into cells is a promising method for treating protein-related diseases, but it is hindered by protein instability in the blood and poor membrane permeability. Effective cellular delivery via systemic administration requires a stable, multifunctional carrier system with cell-targeting and endosomal escape capabilities. In this study, we prepared a catalytic nanoparticle containing an active enzyme by cross-tethering multiple superoxide dismutase (SOD) molecules with catechol-derivatized hyaluronic acid (HA).The structure and catalytic activity of the enzyme molecules in the nanoparticle were not significantly compromised in the nanoparticle.The nanoparticle achieved the efficient hepatic cellular delivery of SOD via a systemic administration and resulted in efficient removal of reactive oxygen species (ROS) in the liver and remarkable improvement of APAP-induced hepatotoxicity and liver injury in animals.