Synthesis of novel duplex-forming oligomers with complementary H-bonding base-pairs
발표자
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초록
내용
Several synthetic polymers have been investigated for discrete self-assembly like DNA, including peptide nucleic acids (PNAs), which utilize hydrogen bonding between nucleobases. However, PNAs are synthesized via solid-phase synthesis, which limits scalability due to the sequential addition of monomers. To address this issue, we propose to use cross-convergent synthesis to synthesize sequence-defined oligoesters with complementary hydrogen-bonding base pairs. The oligoesters have an ester backbone and side chains containing adenine or thymine. By employing orthogonal protecting groups with mutually orthogonal deprotection strategies, we can couple monomers to achieve discrete polyesters with exponentially increasing molecular weights, ultimately synthesizing tetramers. NMR titration experiments will be performed to confirm the formation of stable duplexes. Finally, a sequence library of adenine and thymine will be generated using the cross-convergent method to verify duplex formation between oligomers with complementary sequences.