Magnetically Retrievable Amphiphilic Nanoplatelets as Versatile Supports for Interfacial Enzyme Catalysts
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초록
내용
This study introduces an amphiphilic nanoplatelet (ANPL) system capable of supporting various enzymes as interfacial catalysts. Iron oxide nanoplatelets were synthesized via reverse precipitation of ferrous ions. To prevent aggregation, the nanoplatelets were coated with silica using tetraethyl orthosilicate (TEOS) as a precursor. The surface hydroxyl groups were then modified with amine groups through a silane reaction to attach bromine initiators for polymerization. The silica-coated iron oxide nanoplatelets were simultaneously grafted with hydrophilic poly(2-aminoethylmethacrylate) (pAMA) and hydrophobic poly(n-butylmethacrylate) (pBMA) on opposite sides. Enzyme connectors were formed on the hydrophilic sides of the synthesized ANPLs through imine bonds with glutaraldehyde (GA) to support enzyme catalysts. The activity and cell viability of these enzyme catalysts were evaluated. Notably, these interfacial catalysts could be easily retrieved using an applied magnetic field, enhancing their practicality and reusability.