Extracellular vesicle concentration process development through super absorbent polymer bead-based nanosieve
발표자
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초록
내용
Extracellular vesicles (EVs) are nano-sized vesicles that contain biomarkers originated from their parental cells related with disease. Therefore, they are promising biomaterials in disease diagnosis and drug delivery. For clinical application, EVs must be concentrated or separated from bulk liquid samples, including cell culture media, plant juices, and body fluids. However, conventional EV enrichment methods such as ultrafiltration, ultracentrifugation, and PEG-based precipitation methods are inefficient, time-consuming, and costly, with limited scalability. Here, we describe a super absorbent polymer bead-based nanosieve as an efficient EV enrichment technique. Nanosieve has nano-sized channels that absorb small molecules and water while expelling large molecules, including EVs, to enrich them. Consequently, nanosieve-based EV enrichment can efficiently process a variety of samples, offering time and cost effectiveness, scalability, and the potential for further modifications.