Development of lipid nano particles using bile acid and chondroitin sulfate for drug delivery
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Lipid vesicles have been extensively studied for various applications including drug delivery. Due to amphoteric property of the phospholipids, lipid vesicles can carry both hydrophilic and hydrophobic molecules protecting them from external environment. In this study, we synthesized liposomes using 1,2-Dioleoyl-sn-glycero-3-phosphocholine via thin-film rehydration method followed by extrusion encapsulating green fluorescent protein (GFP) mClover3. Subsequently, glycocholic acid-conjugated chondroitin sulfate (GCA-CS) was synthesized for surface coating of the liposomes. Glycocholic acid is a kind of bile acid that is known to be involved in lipid digestion and uptake process. The size of liposomes was measured by dynamic light scattering. Zeta potential was analyzed to confirm stable coating of GCA-CS. Resulting liposomes were orally injected to the small fry of flatfish. After injection, fractions of the intestine were imaged using fluorecence microscope for quantification of absorbed GFP.