AdMSC spheroids carrying antioxidant hybrid protein carrier for ROS-damaged Salivary Glands Repair
발표자
()
초록
내용
Proteins are critical components for organisms and mediate various biochemical reactions that occur within cells. The novel design of hybrid protein is essential for a successful tissue repair. Herein, hybrid protein was engineered using glutathione (an antioxidant enzyme) and gelatin (a structural protein). The glutathione-gelatin hybrid (GG) performed as an oxidative stress down-regulator. For ROS-damaged salivary gland repair, GG was fabricated into a cell carrier via photo-crosslinking, and adipose-derived mesenchymal stem cell (AdMSC) spheroids were loaded into the GG cell carrier (GGH). The GGH with AdMSC spheroids system was applied to an incurable reactive oxygen species (ROS)-damaged salivary gland mice model. After irradiation, excessive residual ROS were neutralized by GGH, which modulated the pro-inflammatory reaction and tissue fibrosis. Additionally, tissue repair was accelerated by AdMSC spheroids. Antioxidant hybrid protein system is a promising cell/drug carrier for incurable tissue repair.