Biofunctional composite scaffolds containing intermediate mesoderm cells and differentiation-inducing extracellular vesicles for kidney regeneration.
발표자
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초록
내용
Chronic kidney disease (CKD) is a global health issue with irreversible kidney damage, that limited to dialysis and transplantation for treatment. Due to these limitations, research in tissue engineering and regenerative medicine has been actively pursued. However, challenges like low renal cell reproducibility, limited biomaterial bioactivity, and inflammation complicate regeneration still remain. Cellular differentiation shows promising, but kidney complexity and immune responses are obstacles. We developed a porous PMEZ scaffold of PLGA(P), Mg(OH)2(M), ECM(E), and ZnO(Z) to support tissue regeneration. By incorporating extracellular vesicles (EV) derived from human intermediate mesoderm (IM) cells differentiating into kidney cells and IM cells, the scaffold promoted IM cell differentiation into renal lineages in a 3D environment. In a 3/4 nephrectomy CKD mouse model, the PMEZ/dEV/IM scaffold improved tissue regeneration and kidney function, suggesting a promising strategy for CKD treatment.