Electrochemical Monitoring of NADH Redox with NPQD-modified Electrodes based on electropolymerization
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There is increasing interest in the rapid and highly sensitive monitoring of cell viability in biological and toxicological research. This paper reports on the development of a rapid and sensitive electrochemical (EC) sensor using screen printed electrode (SPE) and surface modification using 4’-NPQD, as double electron mediators, for monitoring cell viability via the measurement of nicotinamide adenine dinucleotide (NADH). We used the sensor to observe the viability of MCF-7 and doxorubicin (Dox)-treated cells. The oxidation current of NADH was measured via chronoamperometry (CA), and the EC results showed a good linear relationship when compared with NADH quantification using WST-8 assay. The analysis time was only 10 s and limit of detection (LOD) of NADH was 1.78 μM. Our EC method has the potential to replace conventional WST assays for cell viability and cytotoxicity experiments