Three-dimensional Microenvironment for Enhancing Direct Reprograming
발표자
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초록
내용
Direct reprogramming technology offers a promising therapeutic strategy for tissue regeneration. However, majority of the current in vitro cell culture systems do not provide optimal conditions for the cells to be reprogrammed towards the target lineage. In this talk, I will describe the importance of the tissue-specific microenvironment as the primary mediator of stem cell fate determination. The microenvironment is a complex 3D mixture of biochemically stimulating molecules, biophysical structures, and interacting cells. By understanding the microenvironments participation in regulating cell fate, we could better harness this tool to develop bioengineering technologies. Tissue-specific microenvironment was reconstituted by using tissue-derived extracellular matrices in 3D, providing optimal biochemical and biophysical signals. The resultant target cells displayed more mature cell signatures and ultimately showing improved therapeutic efficacy in vivo. This research was supported by the National Research Foundation of Korea (NRF) grants funded by the Korea government (MSIT) (2021R1C1C2009131).