Real-time visualization of spatiotemporal membrane reconstitution in formation of death-inducing signaling complex
발표자
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초록
내용
A death ligand and sphingomyelinase play a significant role in multimerization of death receptors at cell membranes to initiate downstream apoptosis signaling. The individual functions of the death ligand and the sphingomyelinase are known to guide the death receptor multimers to interact with adaptor proteins for formation of death-inducing signaling complex (DISC) during the intracellular apoptosis signaling. However, it is still unclear how the death ligand and the sphingomyelinase are synergistically activated to spatiotemporally reorganize the cell membranes for the DISC formation. Using a freestanding planar lipid membrane system, in this study, we demonstrate real-time visualization of spatiotemporal membrane reconstitution induced by both the death ligand and the sphingomyelinase in understanding the underlying kinetic mechanism of the DISC formation. This study could provide mechanistic insight into the apoptosis signaling pathway and its relevant therapeutic applications.