Evaluation of click-crosslinked hyaluronic acid hydrogels containing anti-inflammatory peptides for treating rheumatoid arthritis
발표자
()
초록
내용
Rheumatoid arthritis (RA) is an autoimmune disease and patients have to take drug daily. The purpose of this study is to develop an injectable hydrogel that allows drug to be released over a long time so that the drug can act on target site. TLR4-antagonistic peptides (TP) are effective in reducing the levels of pro-inflammatory factors, but have a short half-life in vivo. For sustained release of the peptide, we used hyaluronic acid (HA) and crosslinked it (C-HA) using the click reaction. TAP loaded C-HA (TP+C-HA) can be prepared by just mixing and TP+C-HA exhibits longer peptide release period than TP+HA. As a result, TP+C-HA inhibited the expression of pro-inflammatory cytokines in vitro. TP+C-HA injection into the joint of RA rats showed outstanding therapeutic effects based on histological analysis, and inflammatory factor levels. Intra-articular injection of TP+C-HA can be effective for treating RA by maintaining the therapeutic concentration of peptide for an extended period.