The anion exchange membrane fuel cells (AEMFCs) have recently gained significant interest for their superior advantages over proton exchange membrane fuel cells, such as facilitated redox kinetics. Also, non-aqueous redox flow batteries (NARFBs) are emerging large-scale energy storage systems that can overcome an aqueous system’s inherent low energy density. However, no representative AEMs have been developed until now, satisfying chemical stability, high conductivity, and permselectivity. In these regards, we synthesized the block copolymer-templated nanoporous membrane produced by the polymerization-induced microphase separation (PIMS) with an additional pre-functional middle block introduction. The produced membrane had a bicontinuous mesoporous structure with a densely cross-linked framework. The pre-functional block could be easily converted to quaternary ammonium cationic groups. Detailed characterization of the membranes and the permeability performance will be presented.