Bioprinting of tissue scaffold with hyaluronate-based self-healing hydrogel
발표자
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초록
내용
Extrusion-based three-dimensional (3D) bioprinting has been widely used to fabricate 3D tissue scaffolds containing cells. Bioinks for extrusion-based printing should have the ability to overcome the shear force during extrusion and be sufficiently elastic to build 3D interconnected structures. Herein, hyaluronate (HA)-based hydrogel systems were designed and utilized as 3D bioink. Diol-containing molecules were introduced to HA and partially oxidized to form long chain-oxidized hyaluronate (l-oH). l-oH and hydrazide-modified HA were used to prepare the self-healing hydrogel. Alginate was also added to the hydrogel and crosslinked with calcium ions to enhance the mechanical properties of the hydrogel. The hydrogel showed excellent self-standing ability after 3D printing without frame materials. Various characteristics including tensile properties, self-healing ability, and cell viability were investigated. This hydrogel system also demonstrated the potential as a 3D scaffold to regenerate cartilage tissues.