Dielectrophoretic Separation of Pancreatic Cancer Derived Exosomes using Biomolecule Conjugative Janus Particles
발표자
()
초록
내용
Cancer is a disease caused by the growth of abnormal cells in the body, leading to its decline. Especially pancreatic cancer is difficult to detect in its early stages, leading to a high 5-year death rate. Exosomes have gained attention as a method for early diagnosis of cancers like pancreatic cancer, since they contain information of the underlying cell. Also it has been found that they contain Glypican-1 glycolipids on the cell membrane surface, which binding specific lectins. In this study, we used polystyrene-based Janus Particles (JPs) with both metal layer and lectin-conjugated layer as a solution to distinguish pancreatic cancer-derived exosomes. We also applied microfluidic systems and dielectrophoretic manipulation, and the DEP behaviors of JPs with different lectin and exosome conjugation were checked under various electric field conditions. The results showed the possibility of continuous detection and separation method of exosomes with specific cell membrane composition.