Cationic Hydroxyethyl Cellulose Derivatives for Drug/gene Co-delivery Systems
발표자
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초록
내용
In this study, polyethylenimine (PEI) conjugated hydroxyethyl cellulose (HECP2k) was synthesized and proposed as a promising drug/gene co-delivery systems. Oxidation of hydroxyethyl cellulose using sodium periodate was followed by conjugation with low molecular weight PEI (Mw 2 kDa) to obtain HECP2k. Prior to applying HECP2k to drug/gene co-delivery system, physiochemical and biological characteristics of HECP2k were analyzed. HECP2k formed cationic nanoparticles with pDNA and showed low cytotoxicity toward HeLa and HepG2 cells. HECP2k/pDNA polyplex showed superior transfection efficiency even at high content of serum proteins, indicating its high serum stability. Cellular uptake and intracellular trafficking of HECP2k/pDNA were examined by flow cytometry and confocal microscopy. Finally, encapsulation of doxorubicin and complexation with Bcl-2 siRNA resulted in HECP2k@Dox/siRNA. Size and zeta-potential of HECP2k@Dox/siRNA were measured and antitumor effect was analyzed via MTT assay.