Preparation of uniform gene-loaded lipid nanoparticles using membrane extrusion process
발표자
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초록
내용
mRNA-loaded lipid nanoparticles have been recently developed as a next-generation vaccine platform that is expected to be used as a treatment for not only infectious diseases but also cancer and rare diseases. Lipid nanoparticle (LNP) is a carrier that delivers mRNA containing genetic information and is known to have high delivery efficiency at a size of 100 nm or less. Therefore, various fabrication processes have been actively investigated for uniform LNPs with DNA or RNA. Among them, the bulk mixing method is highly productive, but difficult in controlling the size distribution. Here, we developed a membrane extrusion process for addressing such problem. At first, we prepared relatively large polydisperse gene-loaded LNPs by simply mixing cationic, ionizable, PEG-modified lipids, cholesterol in ethanol and DNA or mRNA in water. Then, through the membrane extrusion process, size distribution of gene-loaded LNP was significantly improved and mean diameter was controlled depending on the pore size of membrane.