Bioprinting of stromal cells-laden artificial cornea using visible light crosslinkable bioinks
발표자
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초록
내용
Bioprinting has the potential for rapid and precise engineering that can mimic the cornea. However, existing bioinks are limited by their poor cytocompatibility, low photo-crosslinking efficiency, and opaque/colored surface. Herein, we report a fast-curable low-energy bioprinting system using a photosensitive hyaluronic acid (HA) and water-soluble photoinitiator (PI) for visible light-induced 3D bioprinting. After examining the water solubility, molar extinction coefficients, photocrosslinking efficiency, and biocompatibility of the PIs, the mechanical properties of bioinks were improved by blending different molecular weight HA by forming multilength networks. Finally, the stromal cell-laden corneal structure was fabricated and the cell viability in the 3D-printed constructs was evaluated. The HA-based photocurable bioinks exhibited superior properties and fast-curing visible light-induced 3D printing material suitable for cell-laden tissue constructs for tissue engineering.