3D printing of magnesium ion-releasing bioink to promote myogenic differentiation
발표자
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초록
내용
With the advancement of three-dimensional (3D) bioprinting technology, studies on the development of novel bioinks have been vigorously introduced. The addition of magnesium ions (Mg2+) is beneficial for effective muscle tissue regeneration because of its promoting effects on cellular behaviors. Herein, we introduce gelatin-based hydrogel bioinks that release Mg2+ to induce spontaneous myogenesis of C2C12 myoblasts. The physicochemical characterization suggested that the prepared bioinks have suitable rheological and mechanical properties to be used in 3D bioprinting. Through the droplet 3D bioprinting technology, highly fine-structured 3D constructs were fabricated. The immunocytochemical analysis showed that the 3D printed constructs facilitated cell proliferation and myosin heavy chain (MHC) expression which is a typical myogenic differentiation marker. In conclusion, we propose that Mg2+‑releasing hydrogel has great potential to be applied for muscle tissue regeneration.